Abstract: To investigate the characteristics of flow field in centrifuge chamber with opening and the effect of opening on wind resistance power, firstly, based on the computational fluid dynamics method, the model for the flow field of a centrifuge chamber is established, and the opening state of the chamber is simulated. Secondly, the tests on the flow field are carried out on a high-speed centrifuge, and the spatial distribution of the pressure and wind speed in the centrifuge room, as well as the change rules with the centrifuge speed, is obtained. The difference of the wind resistance power between the disc rotor and the rotor arm is compared. The results show that the sliding grid technology can be used to calculate the rotating flow field in the centrifuge chamber and the inclined vent is more conducive to the ventilation and heat transfer of the chamber, and reduces the wind resistance power of the centrifuge operation. The rectification of pits on rotating parts can effectively reduce the wind resistance power. The differential pressure resistance of the disc rotor is far less than that of the rotary arm rotor. The heat transfer efficiency of the machine room can be improved by reasonably designing the vent, and the research can provide reference for the design of the machine room and temperature control of the high-speed centrifuge.