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张建伟, 赵聪聪, 尹悦, 石磊, 边汉亮, 韩智光. 紫外诱变产脲酶菌株加固粉土的试验研究[J]. 岩土工程学报, 2023, 45(12): 2500-2509. DOI: 10.11779/CJGE20221076
引用本文: 张建伟, 赵聪聪, 尹悦, 石磊, 边汉亮, 韩智光. 紫外诱变产脲酶菌株加固粉土的试验研究[J]. 岩土工程学报, 2023, 45(12): 2500-2509. DOI: 10.11779/CJGE20221076
ZHANG Jianwei, ZHAO Congcong, YIN Yue, SHI Lei, BIAN Hanliang, HAN Zhiguang. Experimental study on solidification of silt through urease-producing strains induced by ultraviolet mutagenesis[J]. Chinese Journal of Geotechnical Engineering, 2023, 45(12): 2500-2509. DOI: 10.11779/CJGE20221076
Citation: ZHANG Jianwei, ZHAO Congcong, YIN Yue, SHI Lei, BIAN Hanliang, HAN Zhiguang. Experimental study on solidification of silt through urease-producing strains induced by ultraviolet mutagenesis[J]. Chinese Journal of Geotechnical Engineering, 2023, 45(12): 2500-2509. DOI: 10.11779/CJGE20221076

紫外诱变产脲酶菌株加固粉土的试验研究

Experimental study on solidification of silt through urease-producing strains induced by ultraviolet mutagenesis

  • 摘要: 为提高微生物诱导碳酸钙沉淀(MICP)技术的固化效果,采用紫外诱变技术对产脲酶菌株进行改良,筛选出优良菌株。随后结合先拌和菌液后滴注胶结液(尿素和氯化钙)的方式,运用改良后菌株来固化粉土。通过无侧限抗压强度试验、碳酸钙含量测定和微观试验分析,来对比诱变前后菌株固化粉土的效果。结果表明:紫外诱变技术可以有效改良产脲酶菌株的性能,使菌株的脲酶活性、矿化生成的碳酸钙含量得到提高;使用紫外诱变后菌株来固化粉土,可显著提高土体的无侧限抗压强度。该研究从源头来选育优良菌株,有效提高了MICP技术的固化效果。

     

    Abstract: To improve the solidification effects of the microbially induced calcium carbonate precipitation (MICP) technology, the urease-producing strains are optimized by the ultraviolet (UV) mutagenesis. Subsequently, the modified bacterial strains are used to solidify silt by premixing bacterial with soil first and then injecting cementation solution (urea and calcium chloride) into soil. The unconfined compressive strength tests, calcium carbonate content determination and microscopic test analysis are conducted to comparatively study the curing effects of the strains before and after the UV mutagenesis. The results show that the UV mutagenesis technology can effectively improve the performance of the urease-producing bacterial strains, and increase the urease activity and the content of calcium carbonate produced by mineralization. The use of UV-induced bacterial strains to solidify silt can significantly improve the unconfined compressive strength of the soil. This study selects the excellent bacterial strains from the source and effectively improves the curing effects of the MICP technology.

     

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