Abstract: According to the major needs of the national inter-basin water transfer projects in China and the worldwide research situations of related disciplines, the research program focuses on the integrated research and application of construction and safe operation of long-distance water transfer projects. Based on the typical water transfer projects such as the water diversion project in central Yunnan, the water diversion project from Hanjiang River to Weihe River and the tunnel project with large buried depth in Xinjiang. The researches on ten aspects including "Testing technology and comprehensive evaluation method for rock mass characteristics of tunnels with large buried depth" are conducted, aiming at the significant scientific issues such as "response mode and disaster mechanism of excavation of surrounding rock of large buried tunnels", "coordinated bearing mechanism and life-cycle design theory of surrounding rock-support system of large buried tunnels" and "response characteristics and disaster mode of water conveyance buildings under natural disasters such as earthquakes", and the following key technologies: "complete set of disaster prediction and prevention technology of large buried tunnels", "fracture resistance technology of tunnel lining crossing active faults", "seismic mitigation technology of long-span high-rise aqueduct", "development and intelligent control technology of key equipments of gates, pumps and valves", and "life cycle safety monitoring and regulation technology of water transfer projects". The geophysical detection, the testing technology of rock mass characteristic and the evaluation method for characteristics of surrounding rock of deep buried tunnels are put forward. A complete set of technology for the prediction and prevention of high-pressure water inrush in tunnels is formed. A 15 MPa ultra-high pressure grouting technical equipment is developed. The anti-fracture structure of the tunnel crossing an active fault with a displacement of 0.5 m is put forward. A large-scale high-pressure grouting structure in the Ⅷ-degree strong earthquake area is developed. The seismic mitigation technology of aqueduct and the complete set of safety control technology of 220 m-lift and 40 MW-grade-power pump unit and DN2000 intelligent flow-control valve are developed and completed. The relevant research results are applied in some projects, and