Abstract: The soil disturbance deformation induced by penetration of sampler tube has a great influence on the accuracy of physical and mechanical evaluation of soils in the soil investigation engineering. A small-scale model test system is developed to study the non-intrusive measurement of the soil disturbance deformation characteristics during penetration of sampler tube using the transparent soils and PIV (particle image velocimetry) technique. Sampling penetration tests are conducted, and the soil deformation characteristics during penetration of sampler tube are obtained. The test results show that the soil disturbance deformation increases with the penetration depth of sampler tube, and the upheaval deformation of soils outside the sampler is dominated. The disturbance deformation of soils in the sampler is mainly upheaval, and the maximum upheaval deformation occurs at the middle of the sampler in the depth direction. The deformation distribution characteristics of soils on different sections in the sampler are nearly the same, and the shear strain of soils between adjacent sections is relatively small.